41 mg/ml) was obtained from Thermo Fisher Scientific. 5 μL of Binding buffer 2X. To perform the assay, use white plate only. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . For a list of HTRF-compatible readers and set-up recommendations, please visit . It features a modular design that allows users to add or change detection cartridges according to their needs. Make sure the setup for Tb Cryptate is used. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Plate 25 μL of cells in. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. FOR HTRF CERTIFIED. Microplate Readers and Reader Accessories. PHERAstar FSX Powerful and most sensitive HTS plate reader . 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. No washing steps are required. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, AAS System, and dedicated AlphaScreen ® and. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Catalog No. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. LANCE ® Ultra DELFIA ® Certified HTRF Readers and Setups Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. For more. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Incubate 4h at RT. Assay setup for a 384-well low-volume plate. This protocol gives the cells viability and confluence to be monitored. FOR HTRF CERTIFIED READER. HTRF-compatible SpectraMax Multi-Mode Readers. These plates can be read on all HTRF certified readers, thus making their implementation in. These plates can be read on all HTRF certified readers, thus making their implementation in. The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. Conclusion. • HTRF®-Certified Reader. For instance, when assay volumes are. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 3nm, important when reading at 280 nm for DNA analysis, for more exact results. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. best fluorescence polarization plate reader on the market. FOR HTRF CERTIFIED. Push the screw down and turn it clockwise to lock the plate. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. Plate 25 μL of cells in. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Incubate overnight at RT. Your lab might even have one already. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. For more information. Some vendors hold the entire market share for not only a. Preconfigured HTRF. Up to 8 decades dynamic range in one measurement with EDR technology. Plate 25 μL of cells in. 3. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. n HTRF plate validated (QC with reference HTRF assays) n Low to high volumes (10 µL to 350 µL) n Can be used with all HTRF compatible readers n For cell-based or biochemical assays Trying is Believing! Plate Characteristics HTRF® 96 - well low volume plate CulturPlateTM 96 - well Microplate CulturPlate TM 384 - well Microplate ProxiPlateTM 384 - Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key features BMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. For more. CLARIOstar is equipped with a specific optical device which enables the measurement of. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. Dedicated UV-laser excitation. The emission of light by the acceptor will be proportional to the level of interaction. Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Homogeneous Time-Resolved Fluorescence (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based) • Kinases and ATPases • Cytokine Assays TUNE Multi-Mode • cAMP Quantitation • Cell Migration • Cell Viability and Proliferation • Cytotoxicity • DNA/RNA Quantitation (Fluorescent) Homogeneous Time-Resolved Fluorescence (HTRF) . It also integrates with the powerful SoftMax Pro Software for. HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. For a list of HTRF-compatible readers and set-up recommendations, please visit . It can also be further streamlined to a one-. → Incubate 2h (if the detection is not sensitive enough, it is better to incubate 20h). Transcreener® ADP2 FP assay. Transfer 5 µL of cell supernatant from the 96-well plate to a 384-small volume (SV) white detection plate . At Cisbio we provide microplates that help you discover better. A gradient of signal is apparent prior to plate reader optimization (a) causing a %CV of 9. AlphaLISA SureFire Ultra results are expressed as “Alpha counts”, and HTRF results were expressed as DeltaF, calculated according to CisBio instructions. " The PHERAstar FSX instrument is a high quality, reliable, plate reader. + 4 µL HTRF pre-mixed antibodies . Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Read & analyse. These plates can be read on all HTRF certified readers, thus making their implementation in. The ratio of the two fluorescence TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. Plate 25 μL of cells in. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. (665 nm and 620 nm) on a compatible HTRF ® reader. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. com HTRF Microplate Readers. and TR-FRET, for example to detect PerkinElmer’s DELFIA, HTRF and LANCE assay technologies Time resolved fluorescence certified for use with HTRF. • HTRF®-Certified Reader **. (donor) of compounds are measured on an HTRF-compatible reader. Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. Fluorescence polarization (FP) is a fluorescence-based detection method that is widely used to monitor molecular interactions in solution. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 4 mg/ml. 5 nm filter (PerkinElmer 2100-5110) were used. Incubate at room temperature. 96-well culture plate+ 4 µL HTRF pre-mixed antibodies. These plates can be read on all HTRF certified readers, thus making their implementation in. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). In addition, stacker. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Revvity. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for 21 CFR Part 11. 27 / Pack of 100; Qty. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Advanced Phospho-ERK (Thr202/Tyr204) 2-plate assay protocol. Plate 25 μL of cells in. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Revvity. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. For more information about HTRF ®The LUMIstar® Omega is BMG LABTECH's powerful dedicated luminescence microplate reader, providing highly sensitive flash and glow assay readings. The detection antibody is incubated for 1. For more. HTRF reagent concentrations have been set for optimal assay performances. View Brochure. + 4 µL HTRF ® pre-mixed antibodies . CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Each well generally requires 50µL of lysis buffer 1X. The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Incubate 2h at RT Transfer 16 µL supernatant. Synergy NEO2 readers must be appropriately configured for HTRF® readout by setting up the measurement conditions in the Gen5™ Reader Control and Data Analysis Software. + 4 µL HTRF pre-mixed antibodies. It also integrates with the. This protocol gives the cells viability and confluence to be monitored. The multidetection microplate readers CLARIOstar, PHERAstar FS, FLUOstar Omega, and POLARstar Omega are all HTRF-certified by Cisbio, meaning each of the. The donor and acceptor can be used to label a variety of biomolecules, for applications. FOR HTRF CERTIFIED READER For more. FOR HTRF CERTIFIED READER. SpectraMax i3/i3x and Paradigm readers can be equipped with. Read & analyse. . PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. Certified HTRF compatible reader by CisBio Bioassays;Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. For more information about HTRF. The optional HTRF-certified filter-based module may be. Revvity. Make sure the setup for Eu3+ Cryptate is used. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. 14 seconds for a 384-well plate. Data Analysis HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Plate 25 μL of cells in. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For more information about HTRF. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. • ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Transcreener® ADP2 TR-FRET assay. For more information about HTRF. VICTOR Nivo Multimode Plate Reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Both Microplate Optimization and Read Height Adjustment were. Incubate overnight at RT. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Plate 25 μL of cells in. The donor and acceptor can be used to label a variety of biomolecules, for. S. For HTRF readings, a 620/40 nm filter (PerkinElmer 2100-5760) and a 665/7. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The optical systems use two scanningHighly compatible plates for HTRF. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. If not, our scientific team will help you choose the instrument that best suits your needs and budget. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. pre+ 5 µL HTRFcompounds Activation Buffer. Remove the plate sealer and read on an HTRF® compatible reader . The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The plate is set up for reading and images are acquired according to specified parameters. For more information. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF® 96-well low volume plate TECHNOLOGY ALL THE BENEFITS OF HTRF WITH THE EASE OF USE OF AN ELISA PLATE!. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. 5. These are the different kinetic modes on BMG LABTECH microplate readers. This protocol gives the cells viability and confluence to be monitored. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. It can also be further streamlined to a one-. Filters or monochromators for emission wavelength. Make sure the setup for Eu Cryptate is used. For fluorescent assays, black plates absorb light and reduce background and crosstalk. Highly compatible plates for HTRF. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. Even if not listed, some. Cover the plate with a plate sealer. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. Step 1 Step 2 Step 3 Step 4 Step 5. HTRF Setup. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Plate Type and Read Area should be selected based on the microplate used and wells to be read. For more ® information about HTRF compatible readers and for set-up recommendations, please. It can also be further streamlinedFOR HTRF CERTIFIED READER. Due to the chemical and photophysical. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. Using a black. EGFR protein (PV3872, 0. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. Incubate overnight at RT. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. Plate 25 μL of cells in. 96-well culture plateFor each fluorescent ligand concentration, the nonspecific binding was determined by adding a large excess of unlabeled spiperone. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYFOR ®READING, AN HTRF -CERTIFIED READER IS NEEDED. Its. For a list of HTRF-compatible readers and set-up recommendations, please visit . FOR HTRF CERTIFIED READER. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. HTRF Signal + 5 hours @ RT or . 1. For more information about HTRF. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . FOR HTRF CERTIFIED READER. As shown in Fig. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. They can be used in a no-transfer protocol with suspension cells or with. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. 5 µL. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. MATERIALS PROVIDED: KIT . FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER . best fluorescence polarization plate reader on the market. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more information. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Lysis buffer 1X for two-plate assay protocol on adherent cells Determine the amount of lysis buffer needed for the experiment. Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. Make sure the setup for Tb Cryptate is used. FOR HTRF CERTIFIED READER. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Incubate 4h at RT. For more information about HTRF ®Excitation range. For more information about HTRF. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Even if not listed, some readers may be compatible with HTRF technology. Your lab might even have one already. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". For more information about HTRF. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. As shown in Fig. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. + 4 µL HTRF pre-mixed antibodies. HTRF is a proprietary time-resolved fluorescence technology that overcomesThe company offers different HTRF-certified, multidetection microplate readers such as the FLUOstar Omega, POLARstar Omega, CLARIOstar and PHERAstar. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. FOR HTRF CERTIFIED READER For. FOR HTRF CERTIFIED READER. Read & analyse. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. We recommend using a white plate for time-resolved fluorescence assays and other assays where a lower signal is to be expected, to reduce the signal from background autofluorescence. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more ® information about HTRF compatible readers and for set-up recommendations, please. Microplate compatibility is a pivotal importance to the optimization and consistent functionality of any plate reader. (In the following text, the term YTHDC1 is used frequently as an abbreviation for the m 6 A-recognition domain of YTHDC1). and Canaa -8-963-4567. The laser beam passes through the sample well. 47 seconds for a 1536-well plate. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Incubate 24 hours at RT. overnight at 4 C [Prostaglandin E2] pg/ml. Time-resolved fluorescence was measured switch the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Assay setup for a 384-well low-volume plate. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility. COMPONENTS ; STORAGE . • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. This protocol, HTS designed, allows miniaturization while. FOR HTRF CERTIFIED READER For. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. It enables you to gain insights you couldn’t achieve with detection measurements alone. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within theassay linear range 6 Add 4 μL of premixed antibody solutions (vol/vol) prepared in the detection buffer. Remove the plate seal and read on an HTRF compatible reader. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Partnerships for delivering turn-key solutions. Figure 1. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Use simpler, faster protocols. For a list of HTRF-compatible readers and set-up recommendations, please visit . No washing steps are required. FOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in. ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Download Setup. FOR HTRF CERTIFIED. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Remove the plate seal and read on an HTRF compatible reader. Remove the plate seal and read on an HTRF compatible reader. FOR HTRF CERTIFIED READER. On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. HTRF Microplate Readers. The kit includes everything you need: reagents, buffers and a microplate. Compared to ELISA technology, MSD assays: Require far less sample. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. The plate was covered and incubated for two hours at room temperature. For more information about HTRF. 5 EXAMPLE OF MAP PLATE . 96-well culture plateHowever, time-resolved fluorescence (TRF & TR-FRET) assays are used in conjunction with fluorophores that have a longer half-life. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. 96-well culture plateFOR HTRF CERTIFIED READER. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. FOR HTRF CERTIFIED. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Envision Nexus. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Page 30 4. 96-well culture plateHTRF certified . This protocol, HTS designed, allows miniaturization while. For more information. acceptor reagent. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for. + 4 µL HTRF ® pre-mixed antibo dies 2. According to the manufacturers protocol, this protein assay is linear in the range of 0. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Incubate overnight at RT. Incubate overnight at RT. It accepts all types of microtitration plate. No washing steps are required. 0. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation.